Based at the EPFL in Lausanne, BSF-ACCESS provides a complete solution to scientists interested in pursuing chemical screening. The platform offers strong expertise for assay validation, execution and evaluation and large libraries of chemicals and siRNA. Massive screening experiments and the necessary data management and analysis systems to evaluate the results are available. Open laboratory facilities are also accessible to support researchers with specific instruments.
The BSF-ACCESS offer
Services of BSF-ACCESS include consulting, projects advising, guiding and support:
- Chemical screens in 384 and 96 well plates formats for gene knockdown and chemical interference screens.
- Assay development, assay design compatible and amenable to HTS, protocols and SOPs transfer, optimization of experimental conditions.
- Assay automation, development and validation of automation assays, pilot screens with a limited number of compounds, screening scale up.
- Compounds management storage, reformatting, aliquoting, cherry picking and plating of compounds collections.
- Data management using in house developed Laboratory Implementation Management System (LIMS).
Screening at BSF-ACCESS
- Libraries of chemicals and siRNA: A highly diverse chemical library, including the Swiss Chemical collection (SCC from Swiss Chemistry labs) has been established. It comprises approximately 100’000 compounds from various commercial sources including a few specific libraries. This assembly of chemical compounds provides large coverage of the chemical 3D-structural space for drug–like molecules, and contains admitted drugs, man-made compounds as well as natural compounds. A large collection of RNAi’s targeting about 50’000 human and mouse genes allow a detailed and genome-wide interrogation of the involvement of gene products in biological processes of interest.
- The robotic infrastructure of BSF-ACCESS and the appropriate expertise provide support in assay development and automation for the screens. BSF-ACCESS offers a wide variety of multimode readouts for collecting total microtiter-well signal and automated fluorescence microscopy for screening by imaging. Massive screening experiments, such as “high- throughput screens” (HTS) or image-based “high-content screens” (HCS), and the necessary data management and analysis systems to evaluate the results are available.
- Hit confirmation is the final step to validate the hits through more detailed experiments establishing, e.g. dose-responses dependency, and excluding artefacts.
- Open access to infrastructure: BSF ACCESS supports open access to a broad range of fluidic handlers, multimodes readers, automated microscopes and other devices and regularly postgraduate training via formal workshops and seminars.