The Aye lab (LEAGO team) organizes a workshop on measurements and statistical analyses of immune cell viability following electrophile perturbation in developing zebrafish. REX technologies (targetable reactive electrophiles and oxidants (T-REX) and its genome-wide variant (G-REX)) can perturb the immune system with specific reactive small-molecule metabolites in developing zebrafish with precise spatiotemporal and cell-type control. This workshop will take place on October 16, 2019, at the EPFL.
Aims of the workshop
This workshop aims to deliver:
- A brief overview of the REX technologies, including T-REX and G-REX.
- How to use zebrafish as a model organism to study the vetebrate immune system through precision small-molecule perturbation in vivo.
- Video lecture on basic zebrafish techniques including experimental setup, as well as background/standard procedures, such as fish strain maintenance, embryo injection, screening, and evaluation of transgenic marker expression using fluorescence screening of live fish.
- Measurements of fish neutrophil/macrophage levels following electrophile/metabolite modification of a specific protein in vivo.
- Statistical analyses underpinning not only on data interpretation and analysis, but also on how to best design experiments involving precious living models such as choosing the right sample size (appreciation of statisitcal power in data analysis).
- Introduction to REX technologies, including T-REX and G-REX
- Introduction to zebrafish maintenance and experimental setup
- Zebrafish imaging and image quantification assays involving embryos expressing Halo fusion protein
- Fundamentals of statistical analysis
- Deadline to register: October 4, 2019.
- Participants: max. 6 (Master students, PhD and postdoc trainees with interests in quantitative biological research and technologies).
- This workshop is organized by the LEAGO team: Dr. Yi Zhao, Jesse Poganik and Prof. Yimon Aye. Additional co-organizers and workshop assistants: Daniel Urul and Chloé Rogg.
- “T-REX on-demand redox targeting in live cells“, Nat. Protoc., 2016.
- “Akt3 is a privileged first responder in isozyme-specific electrophile response“, Nat. Chem. Bio., 2017.
- “Single-protein-specific redox targeting in live mammalian cells and C. elegans“, Curr. Protoc. Chem. Biol., 2018 .