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Super-resolution microscopy: ready to test your knowledge?

Over the past 10-15 years, new technologies have gone beyond the diffraction limit of optical microscopy. These “super-resolution” microscopies rely on fluorescence imaging and encompass multiple techniques that achieve higher resolution than traditional light microscopy giving exciting opportunities for scientists to ask entirely new levels of questions. The differences between these individual technologies result in a trade-off between spatial resolution, temporal resolution and phototoxicity applied to a specific biological question.

Are you ready to test your knowledge on these microscopy methods used in some of the NCCR labs, such as the Manley lab at the EPFL?

This quiz was created by Victoria von Glasenapp (University of Geneva) and Luis Hernandez Ramirez (EPFL).
Thanks a lot to both of them!

 

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What is the smallest distance between two points that a classical light microscope can distinguish?

Correct! Wrong!

The Nobel Prize for the development of super-resolved fluorescence microscopy was given to:

Correct! Wrong!

Any microscopy technique that overcomes the resolution limit of conventional light microscopy by at least a factor of two is considered to be a super-resolution technique.

Correct! Wrong!

To label the protein of interest in nanoscopy antibodies and immunocytochemistry techniques should be avoided because:

Correct! Wrong!

The following are super-resolution techniques:

Correct! Wrong!

What does Single Molecule Localization Microscopy (SMLM) consist of?

Correct! Wrong!

The following is true about photoactivatable, photoconvertible, and photoswitchable fluorescent proteins, EXCEPT:

Correct! Wrong!

Which of the following statements about Expansion Microscopy (ExM) are true?

Correct! Wrong!

What is the major advantage of Structured illumination microscopy (SIM)?

Correct! Wrong!

Super-resolution microscopy: ready to test your knowledge?
Would you like to increase your knowledge on super resolution microscopy? Read the article written by Prof. Suliana Manley and published on our blog. This article contains a lot of interesting references!
Would you like to know more about this topic? We recommend you to read this article on fluorescence nanoscopy in cell biology published in Nature Reviews Molecular Cell Biology. We hope that it will help you expand your knowledge on these imaging techniques!
You are a true fan of super-resolution microscopy! Congratulations! Discover our other quizzes!

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